Abstract Background Long-read sequencing is revolutionizing genome assembly: as PacBio and Nanopore technologies become more accessible in technicity and in cost. long-read assemblers flourish and are starting to deliver chromosome-level assemblies. However. these long reads are usually error-prone. https://www.roneverhart.com/2-1-2-x-1-4-x-36-Smooth-Saddle-Threshold-NGP-410-36/